Measurement of jet jet substructure in neutral-current deep inelastic ep scattering at high Q2 with ZEUS at HERA Jet substructure for jets produced in deep inelastic ep scattering was studied for exchanged boson virtualities Q2 > 125 GeV2 with the ZEUS at HERA using an integrated luminosity of 370 pb-1 Jets were identified in the laboratory frame using the kT cluster algorithm in the longitudinally invariant inclusive mode. The substructure of jets was investigated in terms of the jet shape and used to highlight differences between quark- and gluon-initiated jets. Leading-logarithm parton-shower Monte Carlo models and next-to-leading-order QCD calculations are compared to the data and describe the measurements well.